專任教授(植物暨微生物環境生物學組)-中央大學生命科學系資訊網

STAFF系所成員

首頁系所成員專任教授(植物暨微生物環境生物學組)陸重安 博士

系所成員

專任教授(植物暨微生物環境生物學組)

  • 發佈日期: 2019-08-21
  • 最後更新日: 2024-08-01
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陸重安 博士

  • 職稱: 特聘教授
  • 學歷: 國防大學
  • 專長: 植物訊息傳導、植物基因調控、植物組織培養
  • 信箱: chungan@cc.ncu.edu.tw
  • 分機: 65067、65077
現職與學經歷
  • 現  職：
  1. 國立中央大學 生命科學系 教授
  • 學  歷：
  1. Ph.D ：National Defense Medical Collage, Institute of Life Science (1992~1998)
  2. M.S. ：Chinese Culture University, Institute of Biotechnology. (1990~1992)
  3. B.S.  ：Chinese Culture University, Department of Botany. (1986~1990)
  • 經  歷：
  1. Assistant Professor, Dept. of Life Sciences, Natl. Central Univ. (2004~2017)
  2. Postdoctoral Fellow, with Dr. Karen E. Koch, Horticultural Sciences Department, University of Florida. Research on (1) the relationship between ZmCAF1 and poly(A) tail; (2) functional genomics in Maize. (2003~2004)
  3. Visiting Postdoctoral Fellow, with Dr. Tuan-Hua David Ho, Department of Biology, Washington University, St. Louis. Research on sugar and hormonal regulation of a-amylase gene expression. (2000~2000)
  4. Postdoctoral Fellow, with Dr. Su-May Yu, Institute of Molecular Biology, Academia Sinica. Research on mechanism of sugar-dependent transcriptional control of a-amylase gene expression. (1998~2003)





授課與研究領域

授課

  1. 分子生物學
  2. 植物訊息傳遞
  3. 植物型態與解剖
  4. 專題：mRNA穩定度

研究領域

Molecular mechanism of RNA metabolism in regulating gene expression in plant development.



a. Functional analysis of DEAD-box proteins in plants

RNA(包括mRNA、rRNA與tRNA)分子一直扮演著重要的角色，正確的RNA分子及它們所形成特定的二級與三級結構，影響著後續特定基因的正常表現，與它們所扮演的生理功能。RNA helicases是細胞內負責維持與重組正確RNA分子結構的主要酵素，它能解開雙股RNA，重組RNA結構，進而影響RNA的功能。DEAD box RNA helicases是RNA helicases家族中成員最大的一族，它幾乎參與了所有和RNA分子有關的一些細胞生理過程，包括transcription、nuclear RNA splicing、RNAtransport、RNA turnover、RNAi、RNA editing、translation initiation與 ribosome biogenesis。雖然DEAD box RNA helicases參與這些不同的細胞生理過程，但是它們的確切的功能與調控方式仍需要進一步釐清。目前普遍使用酵母菌來研究DEAD box RNA helicases，但是植物(以水稻與阿拉伯芥為例)屬於高等多細胞真核生物有著不同的特化細胞，這些細胞必須協調運作以維持完整生命週期。因此，只以單細胞酵母菌的研究來推測DEAD box RNA helicases在植物體中的角色常常不能呈現它完整的面貌。為了瞭解植物DEAD box RNA helicases的真正功能、調控方式與影響層面，我們探討對植物中不同DEAD box RNA helicase的功能。首先我們已經對植物中DEAD box RNA helicases-36 (RH36)的初步研究成果，並逐步解開它在植物中的所扮演的角色，我們的結果已經確定RH36 功能與植物雌配子體有關。


b. Poly(A) tail and gene expression in plant

基因表現的調控機制可分為轉錄階層 (transcriptional level) 和後轉錄階層 (post-transcriptional) 兩種形式；然而人們對於基因表現在轉錄階層的調控機制較為熟悉，對於後轉錄階層的調控則較為陌生。在真核生物中，mRNA的降解過程是poly(A) tail先被分解，接著才進行5’ decapping和5’ exonuclease催化的5'到3'方向的mRNA分解，或不經由5’decapping而是直接進行3' exonuclease催化的3’到5’方向的mRNA分解，而poly(A) tail的分解是絕大部分mRNA降解的速率決定步驟。從酵母菌的研究中得知，CAF1 (CCR4 associated factor 1) 能與CCR4 (carbohydrate catabolism repression 4) 結合並參與mRNA的代謝。目前已經知道CAF1與CCR4這兩個蛋白質具有deadenylase的功能，所以一般相信它們直接參與了mRNA的分解，然而我們並不了解CAF1與CCR4基因在植物系統中是否具有相同的功能。我們選擇單子葉模式植物-水稻為基礎來分析CAF1與CCR4的功能。我們分離出水稻的CAF1與CCR4基因，然後分析基因的表現，釐清水稻中各別CAF1與CCR4基因在不同組織或器官中的表現模式。我們發現其中OsCAF1A表現在Leaves、Shoots、Sheaths與Panicles，而OsCAF1B表現在Roots、Leaves、Nodes、Senesces leaves與Panicles；另外，OsCAF1A是受糖抑制表現的基因，而OsCAF1B為受糖誘導表現的基因。此外利用E.Coli表現系統將OsCAF1A、 OsCAF1B重組蛋白大量的表達出來，並確定其都具deadenylase的功能。最後我們也已經構築完成OsCAF1A 與 OsCAF1B over-expression 及RNAi的表達載體，並使用基因轉殖方法將OsCAF1A和OsCAF1B在水稻中大量表現，另一方面進行RNAi抑制水稻內生OsCAF1基因的表現，希望藉由這些水稻轉殖植株，可以進一步了解CAF1基因在水稻中所扮演的角色。




論文發表

Publications                                                 

  1. Nguyen TM., Lu CA*, Huang LF* (2021) Applications of CRISPR/Cas9 in a rice protein expression system via an intron-targeted insertion approach. (https://doi.org/10.1016/j.plantsci.2021.111132).
  2. Sinaga DS., Ho SL., Lu CA, Yu SM, Huang LF* (2021) Knockdown expression of a MYB-related transcription factor gene, OsMYBS2, enhances production of recombinant proteins in rice suspension cell. Plant Methods, 17, 99.
  3. Wang TY, Wu JR, Duong NKT, Lu CA, Yeh CH, Wu SJ* (2021) HSP70-4 and farnesylated AtJ3 constitute a specific HSP70/HSP40-based chaperone machinery essential for heat stress tolerance in Arabidopsis. JOURNAL OF PLANT PHYSIOLOGY, 261 113543.
  4. Fang JC, Tsai YC, Chou WL, Liu HY, Chang CC, Wu SJ, Lu CA* (2021) A CCR4-associated factor 1, OsCAF1B, confers tolerance of low-temperature stress to rice seedlings. Plant Mol Biol. 105, 177-192.
  5. Hsieh PH, Chung YH, Lee KT, Wang SY, Lu CA, Hsieh MH* (2021) The rice PALE1 homolog is involved in the biosynthesis of vitamin B1. Plant Biotechnol J. 2020, 1-3.
  6. Fang JC, Liu HY, Tsai YC, Chou WL, Chang CC, Lu CA* (2020) A CCR4 association factor 1, OsCAF1B, participates in the αAmy3 mRNA poly(A) tail shortening and plays a role in germination and seedling growth. Plant Cell Physiol. 61, 554-564.
  7. Lu CA*, Huang CK, Huang WS, Huang TS, Liu HY, Chen YF (2020) DEAD-Box RNA Helicase 42 Plays a Critical Role in Pre-mRNA Splicing under Cold Stress. Plant Physiol. 182, 255-271.
  8. Chen YS, Ho Tuan-Hua David , Liu L, Lee DH, Lee CH, Chen YR, Lin SY, Lu CA*, Yu SM*, (2019) Sugar starvation-regulated MYBS2 and 14-3-3 protein interactions enhance plant growth, stress tolerance, and grain weight in rice. PNAS. 116 (43): 21925-21935.
  9. Chen YS, Chao YC, Tseng TW, Huang CK, Lo PC, Lu CA* (2017) Two MYB-related transcription factors play opposite roles in sugar signaling in Arabidopsis. Plant Mol Biol. 93(3):299-311.
  10. Chou WL, Chung YL, Fang JC, Lu CA* (2017) Novel interaction between CCR4 and CAF1 in rice CCR4-NOT deadenylase complex. Plant Mol Biol. 93(1-2):79-96.
  11. Huang CK, Sie YS, Chen YF, Huang TS, Lu CA* (2016) Two highly similar DEAD box proteins, OsRH2 and OsRH34, homologous to eukaryotic initiation factor 4AIII, play roles of the exon junction complex in regulating growth and development in rice. BMC Plant Biol. 12;16(1):84.
  12. Huang CK, Shen YL, Huang LF, Wu SJ, Yeh CH, Lu CA* (2016) The DEAD-box RNA helicase AtRH7/PRH75 participates in pre-rRNA processing, plant development and cold tolerance in Arabidopsis. Plant Cell Physiol. 57 (1): 174-191.
  13. Wu TM, Lin KC, Liau WS, Chao YY, Yang LH, Chen SY, Lu CA, Hong CY* (2016). A set of GFP-based organelle marker lines combined with DsRed-based gateway vectors for subcellular localization study in rice (Oryza sativa L.). Plant Mol Biol. 90(1-2):107-15.
  14. Huang LF*, Tan CC, Yeh JF, Liu HY, Liu YK, Ho SL, Lu CA* (2015) Efficient Secretion of Recombinant Proteins from Rice Suspension-Cultured Cells Modulated by the Choice of Signal Peptide. PLoS ONE 10(10): e0140812
    (SCI; IF: 3.234; Ranking:9/57 in Multidisciplinary Sciences)
  15. Huang CK, Lo PC, Huang LF, Wu SJ, Yeh CH, Lu CA* (2015) A single-repeat MYB transcription repressor, MYBH, participates in regulation of leaf senescence in Arabidopsis. Plant Mol Biol. 88:269-86.
    (SCI; IF: 4.257; Ranking: 17/204 in Plant Sciences)
  16. Chen YS, Lo SF, Sun PK, Lu CA, Ho TH*, Yu SM* (2014) A late embryogenesis abundant protein HVA1 regulated by an inducible promoter enhances root growth and abiotic stress tolerance in rice without yield penalty. Plant Biotechnol J. 13:105-16.
    (SCI; IF: 5.752; Ranking: 11/204 in Plant Sciences)
  17. Chou WL, Huang LF, Fang JC, Yeh CH, Hong CY, Wu SJ, Lu CA*. (2014). Divergence of the expression and subcellular localization of CCR4-associated factor 1 (CAF1) deadenylase proteins in Oryza sativa. Plant Mol Biol. 85:443-58.
    (SCI; IF: 4.257; Ranking: 17/204 in Plant Sciences)
  18. Hsu KH, Liu CC, Wu SJ, Kuo YY, Lu CA, Wu CR, Lian PJ, Hong CY, Ke YT, Huang JH, Yeh CH*. (2014). Expression of a gene encoding a rice RING zinc-finger protein, OsRZFP34, enhances stomata opening. Plant Mol Biol. 86:125-37.
    (SCI; IF: 4.257; Ranking: 17/204 in Plant Sciences)
  19. Lin CR, Lee KW, Chen CY, Hong YF, Chen JL, Lu CA, Chen KT, Ho TH, Yu SM*. (2014). SnRK1A-interacting negative regulators modulate the nutrient starvation signaling sensor SnRK1 in source-sink communication in cereal seedlings under abiotic stress. Plant Cell. 26:808-27.
    (SCI; IF: 9.338; Ranking: 4/204 in Plant Sciences)
  20. Ho SL , Huang LF, Lu CA, He SL, Wang CC, Yu SP, Chen J, Yu SM* (2013). Sugar starvation- and GA-inducible calcium-dependent protein kinase 1 feedback regulates GA biosynthesis and activates a 14-3-3 protein to confer drought tolerance in rice seedlings. Plant Mol Biol. 81:347-361.
    (SCI; IF: 4.257; Ranking: 17/204 in Plant Sciences)
  21. Wang LC, Wu JR, Chang WL, Yeh CH, Ke YT, Lu CA, Wu SJ* (2013). Arabidopsis HIT4 encodes a novel chromocentre-localized protein involved in the heat reactivation of transcriptionally silent loci and is essential for heat tolerance in plants. J Exp Bot. 64:1689-1701.
    (SCI; IF: 5.526; Ranking: 12/204 in Plant Sciences) 
  22. Kuo YC, Tan CC, Ku JT, Hsu WC, Su SC, Lu CA, Huang LF * (2013). Improving Pharmaceutical Protein Production in Oryza sativa. Int J Mol Sci. 14: 8719-8739.
    (SCI; IF: 2.286; Ranking: 46/157 in Chemistry, Multidisciplinary)
  23. Hong YF, Ho TH, Wu CF, Ho SL, Yeh RH, Lu CA, Chen PW, Yu LC, Chao A, Yu SM* (2012). Convergent Starvation Signals and Hormone Crosstalk in Regulating Nutrient Mobilization upon Germination in Cereals. Plant Cell. 24: 2857–2873.
    (SCI; IF: 9.338; Ranking: 4/204 in Plant Sciences)
  24. Liu YK*, Huang LF, Ho SL, Liao CY, Liu HY, Lai YH, Yu SM, Lu CA* (2012) Production of mouse granulocyte-macrophage colony stimulating factor by Gateway technology and transgenic rice cell culture. Biotechnol Bioeng. 109:1239-1247.
    (SCI; IF: 4.126; Ranking: 24/163 in Biotechnology & Applied Microbiology)
  25. Lu KJ, Huang NC, Liu YS, Lu CA, Yu TS* (2012) Long-distance movement of Arabidopsis FLOWERING LOCUS T RNA participates in systemic floral regulation. RNA Biology 9:653-662.
    (SCI; IF: 4.974; Ranking: 52/290 in Biochemistry & Molecular Biology)
  26. Huang CK, Yu SM, Lu CA* (2010) A rice DEAD-box protein, OsRH36, can complement an Arabidopsis atrh36 mutant, but cannot functionally replace its yeast homolog Dbp8p. Plant Mol Biol. 74:119-128.
  27. Huang CK, Huang LF, Huang JJ, Wu SJ, Yeh CH, Lu CA* (2010) A DEAD-box protein, AtRH36, is essential for female gametophyte development and is involved in rRNA biogenesis in Arabidopsis. Plant Cell Physiol. 51(5):694-706.
  28. Wu SJ, Wang LC, Yeh CH, Lu CA, Wu SJ* (2010) Isolation and characterization of the Arabidopsis heat-intolerant 2 (hit2) mutant reveal the essential role of the nuclear export receptor EXPORTIN1A (XPO1A) in plant heat tolerance. New Phytol. 186(4):833-842.
  29. Guan JC, Yeh CH, Lin YP, Ke YT, Chen MT, You JW, Liu YH, Lu CA, Wu SJ, Lin CY*. (2010) A 9 bp cis-element in the promoters of class I small heat shock protein genes on chromosome 3 in rice mediates L-azetidine-2-carboxylic acid and heat shock responses. J Exp Bot. 61(15):4249-4261.
  30. Su CF, Wang YC, Hsieh TH, Lu CA, Tseng TH, Yu SM* (2010) A Novel MYBS3-dependent Pathway Confers Cold Tolerance in Rice. Plant Physiol. 153(1):145-58.
  31. Lee KW, Chen PW, Lu CA, Chen S, Ho TH.D. and Yu SM* (2009) Coordinated Responses to Oxygen and Sugar Deficiency Allow Rice Seedlings to Tolerate Flooding. Sci Signal. 6 October 2009: ra61.
  32. Wang LC, Yeh CH, Sayler RJ, Lee YY, Lu CA, Wu SJ* (2008) Arabidopsis HIT1, a putative homolog of yeast tethering protein Vps53p, is required for pollen tube elongation. Botanical Studies 49:25-32.
  33. Lu CA, Lin CC, Lee KW, Chen JL, Huang LF, Ho SL, Liu HJ, Hsing YI, Yu SM* (2007) The SnRK1A Protein Kinase Plays a Key Role in Sugar Signaling during Germination and Seedling Growth of Rice. Plant Cell. 19:2484-2499.
  34. Huang LF, Liu YK, Lu CA, Hsieh SL, Yu SM* (2005) Production of Human Serum Albumin by Metabolically Regulated Plant Cell Cultures. Transgenic Res. 14:569-81.
  35. Yong W, Link B, O'Malley R, Tewari J, Hunter CT, Lu CA, Li X, Bleecker AB, Koch KE, McCann MC, McCarty DR, Patterson SE, Reiter WD, Staiger C, Thomas SR, Vermerris W, Carpita NC (2005) Genomics of plant cell wall biogenesis. Planta. 221:747-51.
  36. Lee YC, Lu CA, Casaretto J, Yu SM (2003) A bZIP protein, OsBZ8, Mediates Sugar Repression of a-Amylase Gene Expression. Physiol Plant. 119:78-86.
  37. Lu CA, Ho TH D, Yu SM (2002) Three novel MYB proteins with single DNA-binding repeats mediate sugar and hormonal regulation of a-amylase gene expression. Plant Cell 14: 1963-1980.
  38. Chen PW, Lu CA, Yu TS, Tseng, TH, Wang CS, Yu SM (2002) Rice a-amylase transcriptional enhancers direct multiple mode regulation of promoters in transgenic rice. J Biol Chem 277: 13641-13649.
  39. Lu CA, Lim EK, Yu SM (1998) Sugar response sequence in the promoter of a rice a-amylase gene serves as transcriptional enhancer. J Biol Chem 273:10120-10131.
榮譽事項
  1. Outstanding Research Award, National Central University (2010) 99學年度中央大學研究傑出獎
  2. Best Research Paper Award for Top Journal Publication at 2008 College of Science, National Central University 97年度中央大學理學院頂尖期刊論文獎
  3. “The SnRK1A Protein Kinase Plays a Key Role in Sugar Signaling during Germination and Seedling Growth of Rice” was recommended by Faculty of 1000 Biology in 2007 (as the first author of this Pant Cell paper).2007年以第一作者發表於Plant Cell 期刊之論文，獲國際生物學論文評選組織 Faculty of 1000 / Biology 年度推薦文章
學會會員
  1. American Society of Plant Biologists
  2. The Japanese Society of Plant Physiologists
  3. The Botanical Society of Republic of China (中華植物學會)
  4. The Chinese Society of Cell and Molecular Biology (中華民國細胞及分子生物學學會)
實驗室成員

  • 助理：劉欣怡
  • 博士班學生：方振丞
  • 碩士班學生：梁凱茵


外籍博士班學生 Foreign PhD student

Name Country of Citizenship E-mail
阮武保Nguyen Vu Bao
 Vietnam nguyenvubao@dhsptn.edu.vn
阮氏美Nguyen Thi Mai Vietnam nguyenthimai87@tuaf.edu.vn
辛娜嘉Desyanti Saulina Sinaga Indonesia 108881603@cc.ncu.edu.tw


外籍碩士班學生 Foreign master student

Name Country of Citizenship E-mail
弗琳娜AFRILINA SINAGA Indonesia afrilinasinaga25@gmail.com


===畢業學生===

黃俊凱 (2011) 方振丞 (2011) 葉如芳 (2011) 曾梓維 (2011) 陳逸詩(2019)
林惠文 (2009) 吳珮綺 (2009) 賴穎慧 (2009) 吳欽鋒 (2009) 何浩恩 (2009) 陳麗雅 (2009)
李宗樺 (2008) 馬翎甄 (2007) 劉馨如 (2006) 黃偉哲 (2006) 黃俊凱 (2006) 趙志豪 (2006)

 


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