Full-time Professors (Plant, Microbial and Environmental Biology)-Department of Life Sciences, NCU

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Full-time Professors (Plant, Microbial and Environmental Biology)

  • Release: 2022-02-10
  • Update: 2024-04-18
  • Source：
  • Visits：1833

Dr. Shaw-Jye Wu

  • Job title: Professor
  • Education: Department of Botany and Microbiology, Auburn University, USA
  • Expertise: Plant Physiology, DNA Computing, Plant Stress, Biotechnology
  • mailbox: jyewu@cc.ncu.edu.tw
  • Extension: 65052
Introduction
  1. Position: Professor, Dept. of Life Sciences, Natl. Central Univ.
  2. Education:
    1. Ph. D. Department of Botany and Microbiology Auburn University, AL 36849 Dissertation : Molecular genetic approach to the study of stress reponases in Arabidopsis. Dec. 1997
    2. M.S. Department of Botany and Microbiology Auburn University, AL 36849 Thesis title : Search for sigma factor 54 in Frankia .Aug. 1994
    3. B.A. Department of Botany National Taiwan University Taipei, Taiwan Jul. 1989
  3. Professional Experience:
    1. 2015/8-present: Professor, Dept. of Life Sciences, Natl. Central Univ.
    2. 2011/8-2015/7: Associate Professor, Dept. of Life Sciences, Natl. Central Univ.
    3. 2002-2011/7: Assistant Professor, Dept. of Life Sciences, Natl. Central Univ.
    4. Postdoctoral Research Associate (2000 - to 2002) Plant Pathology Department, University of California at Davis Project: "Application of DNA macro array in the detection and identification of pathogens"
    5. Postdoctoral Research Associate (1998 - 2000) Department of Biological Sciences, Auburn University Project: "Regulation of Arabidopsis Glutamate Decarboxylase (GAD) in Response to Heat Stress."
  4. Current Position: 2015/8-present: Professor, Dept. of Life Sciences, Natl. Central Univ.
  5. Contact:886-3-422-7151 ext 65052
  6. FAX:886-3-422-8482
  7. E-mail: jyewu@cc.ncu.edu.tw
Research

Molecular genetic approach to the study of stress responses in plants

Temperature is one of the most ever-changing environmental factors and heat stress derived from elevated temperature can have detrimental impact on living organisms.  Plants, being immobile and unable to escape from their habitat, are more vulnerable to such adverse conditions.  This has made heat stress one of the principal factors that limits crop production worldwide.  To find the genetic determinants that play important role in plant survival under heat stress, with the ultimate goal of improving stress tolerance of crops, a novel genetic approach was proposed to directly identify genes that are essential in stress defense.  The proposed strategy is to identify Arabidopsis thaliana mutants that are hypersensitive to stress, since mutations in genes that are essential for plant to cope with stresses would result in the hypersensitive phenotypes.
Several heat-hypersensitive mutants were selected from an ethylmethane sulfonate mutagenized seed pool.  Among them, hit1-1 is hypersensitive to sustained high temperature.  The mutated gene was later identified, through a map-based cloning procedure, to encode a homolog of the yeast tethering factor Vps53p protein (Lee et al., 2006; Wang et al., 2008).  This new finding is highly recognized as our paper reporting HIT1 is selected by Faculty of 1000/Biology as a “must read” article  (http://www.f1000biology.com/article/id/1031236/evaluation).  Clearly, further characterization and analysis of the function of HIT1 should provide more insights into the importance of vesicle trafficking in plant stress responses, and we are now making efforts to this end. 
Besides, we have shown that hit2 is a nonsense mutation of Arabidopsis XPO1A, which encodes a nuclear transport receptor. These results revealed that XPO1A is functionally distinguishable from its paralogue XPO1B, and XPO1A is specifically required for plant survival under various stress conditions (Wu et al., 2010).  The isolation and characterization of hit2 indubitably opened a brand new frontier for the research community to explore the complex responses of plants to heat stress at the level of regulation of XPO1A-mediated nucleo-cytoplasmic trafficking of macromolecules.  We are currently pursuing the identity of the molecule whose pattern of nucleo-cytoplasmic partitioning through XPO1A must change in order for plants to survive under heat stress.  
There are several other heat-intolerant mutants that have been isolated in our laboratory and we are now mapping the hit3 and hit4 loci.  In the long run, by screening and studying more hit mutants, our research can unveil more fundamental knowledge in plant biology as well as provide practical application in agriculture.    
Paper

  1. Liao ZH, Shih HT, Dong YJ, Tseng MJ, Wang SH, Chen SJ, Wu SJ*, Huang RN*. (2024) Insecticidal action of mammalian galectin-1-transfected Arabidopsis thaliana. Pest Management Science, epub on 30 march 2024, https://doi.org/10.1002/ps.8109 (SCI, 9/100, engomology). 本人為通訊作者.

  2. Wu JR, Zohra R, Duong NKT, Yeh CH, Lu CA, Wu SJ*. (2023, Oct). A plant protein farnesylation system in prokaryotic cells reveals Arabidopsis AtJ3 produced and farnesylated in E. coli maintains its function of protecting proteins from heat inactivation. Plant Methods, 19:113. (SCI, 33/239, Plant Sciences). nstc 111-2311-B-008-001. 本人為通訊作者.

  3. Tseng TS, Rajendran SK, Liu YH, Wu SJ, Lu CA, Yeh CH*. (2023, Aug). Overexpression of OsHsp 18.0 in rice enhanced tolerance to heavy metal stress. Plant Cell Reports, 42, 1841–1843. (SCI, 24/239, Plant Sciences).

  4. Cai-N Cheng, Shaw-Jye Wu*, Andrew Chih Wei Huang* (2022, May). Environmental Enrichment Components Required to Reduce Methamphetamine- Induced Behavioral Sensitization in Mice:Examination of Behaviors and Neural Substrates. Journal of Clinical Medicine, 11:3051. (SCI). nstc 110-2410-H-431-004. 本人為通訊作者.

  5. Wang TY, Wu JR, Duong NKT, Lu CA, Yeh CH, Wu SJ* (2021, Jun). HSP70-4 and farnesylated AtJ3 constitute a specific HSP70/HSP40-based chaperone machinery essential for prolonged heat stress tolerance in Arabidopsis. Journal of Plant Physiology, 261:153430. (SCI, 55/235). MOST 108-2311-B-008-004- MY3. 本人為通訊作者.

  6. Shin-Yuan Gu, Wan-Sheng Lo, Shaw-Jye Wu, Long-Chi Wang (2021, Mar). Dimerization of the ETO1 family proteins plays a crucial role in regulating ethylene biosynthesis in Arabidopsis thaliana. Plant Journal, 105:1293-1308. (SCI, 15/235, Plant Science).

  7. Fang JC, Tsai YC, Chou WL, Liu HY, Chang CC, Wu SJ, Lu CA. (2021, Jan). A CCR4-associated factor 1, OsCAF1B, confers tolerance of low-temperature stress to rice seedlings. Plant Molecular Biology, 105:177-192. (SCI, 42/234 , Plant Science).

  8. Cai-N Chenga, Andrew Chih Wei Huang*, Shaw-Jye Wu* (2020, Jan). Roles of nucleus accumbens shell and core in footshock-induced stress altering behavioral sensitization by methamphetamine in acquisition and testing . Behavioural Brain Research, 380:112434. (SCI, 16/53, Behavioral Sciences). MOST 107-2410-H- 431-004. 本人為通訊作者.

  9. Jia-Rong Wu, Tzu-Yun Wang, Chi-Pei Weng, Ngoc Kieu Thi Duong & Shaw-Jye Wu* (2019, Nov). AtJ3, a specific HSP40 protein, mediates protein farnesylation-dependent response to heat stress in Arabidopsis. Planta, 250:1449-1460. (SCI, 40/235, Plant Science). MOST 105-2311-B-008-004-MY3. 本人為通訊作者.

  10. Kuan-Fu Lin, Meng-Yu Tsai, Chung-An Lu, Shaw-Jye Wu, Ching-Hui Yeh* (2018, May). The roles of Arabidopsis HSFA2, HSFA4a,and HSFA7a in the heat shock response and cytosolic protein response. Botanical Sutdies. (SCI, 113/223, Plant Sciences).

  11. Huang HY, Chang KY, Wu SJ* (2018, Jan). High irradiance sensitive phenotype of Arabidopsis hit2/xpo1a mutant is caused in part by nuclear confinement of AtHsfA4a.. Journal of Biologia Plantarum, 62 (1): 69-79. (SCI). MOST 105- 2311-B-008-004-MY3. 本人為通訊作者.



Teaching
  1. Plasmid
  2. Plant Physiology
  3. Case study of advanced biotechnology
  4. Introduction to computer s science
Book
  1. Lian-Chin Wang, and Shaw-Jye Wu. The EXPORTIN1 gene (XPO1A and XPO1B) in Arabidopsis- Are they functionally redundant? In Nuclear Receptors (Margaret K. Bates et al, ed.), Nova Science Publishers Chepter7.
Laboratory members
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