Full-time Professors (Biomedicine)-Department of Life Sciences, NCU

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Full-time Professors (Biomedicine)

  • Release: 2022-02-10
  • Update: 2023-08-31
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  • Visits：1862

Dr. Pei-Yi Wu

  • Job title: Assistant Professor
  • Education: Department of Life Science, College of Life Science, National Taiwan University, Taipei, Taiwan
  • Expertise: Cell biology; Cancer biology; Histology; Anatomy
  • mailbox: pywu@ncu.edu.tw
  • Extension: 65086
Introduction
  1. Position: Assistant Professor
  2. Education:
    1. Ph.D., Department of Life Science, College of Life Science, National Taiwan University, Taipei, Taiwan, December 2013.
    2. M.S., Department of Cell Biology and Anatomy, College of Medicine, National Chung Kung University, Tainan, Taiwan, June 2005.
    3. B.S., Biology, National Chung Kung University, Tainan, Taiwan, June 2003.
  3. Professional Experience:
    1. 2020/02-present： Assistant Professor, Department of Life Sciences, National Central University
    2. 01/2015- 01/2020   Post-Doctoral Fellow, Institute of Cellular and Organismic Biology, Academia Sinica.
    3. 11/2018 - 04/2019   Visiting Researcher, Research Center for Dynamic Living Systems, Graduate School of Biostudies, Kyoto University.
    4. 01/2014 - 12/2014   Post-Doctoral Fellow, Department of Electrical and Engineering and Department of Life Science, Nation Taiwan University.
    5. 02/2011 - 07/2011   Adjunct Instructor, Department of Natural Science, Taipei Municipal University of Education.
    6. 09/2007 - 12/2013   Teaching Assistant, Nation Taiwan University.
  4. Contact:886-3-422-7151 ext 65086
  5. FAX:(03) 422-8482
  6. E-mail:pywu@ncu.edu.tw
Research

1.Neuroblastoma:


Neuroblastoma (NB) is a highly malignant pediatric cancer derived from the sympathoadrenal lineage of the neural crest during embryonic development. Amplification of the oncogene MYCN is closely associated with poor prognosis of NB. Our recent study found that overexpression of aryl hydrocarbon receptor (AHR) downregulates MYCN expression and promoting cell differentiation. The clinical analysis also show that positive AHR expression strongly correlated with differentiated histology of NB and predicted better survival for patients. In addition, overexpression of AHR significantly suppressed NB xenograft tumor growth. All these findings suggested that AHR is an important mediator of NB pathology, and activation of AHR signaling may offer a new therapeutic possibility for NB patients. Since AHR is a ligand activating transcription factor, we tried to use its endogenous ligands, kynurenine (Kyn) and tetrahydrocorticosterone (THB), for translational medicine experiments. The preliminary data showed that activation of AHR by the endogenous ligands affects several tumor cell behavior of NB in vitro, including promotion of cell differentiation and adhesion and inhibition of proliferation and migration. In the future study, we would like to further investigate the therapeutic potential of AHR and its ligands in NB treatment. In addition, the role of AHR signaling on the tumorigenesis of NB will be examined. All the information obtained from the study will provide important information for developing new therapeutic strategy for NB.

2.Alzheimer’s disease:


Amyloid beta (Aβ) deposition was considered as a major cause of neurotoxicity in Alzheimer’s disease (AD). Aβ peptides are produced by stepwise cleavages of amyloid precursor protein (APP) by β- and γ-secretase. Thus, targeting γ-secretase to suppress Aβ production became one of the therapeutic approaches toward treatment of AD in the past decade. However, more than 90 endogenous substrates of γ-secretase, including Notch, have been identified which indicates pharmacological inhibition of γ-secretase may cause extensive side effects besides the inhibitory effect on Aβ production. Therefore, exploring γ-secretase modulators that selectively suppresses APP processing without altering other substrates becomes an alternative strategy for the development of AD therapeutics. To this end, we established a cell based γ-secretase modulator selection platform and successfully identified some candidates. The therapeutic potential of these γ-secretase modulator will be carefully verified in the future study. Currently, we have known that ErbB2 possess the ability to regulate APP processing by mediating autophagy without affecting Notch signaling. Inhibition of ErbB2 significantly alleviates the production of Aβ and renders cognitive improvement in APP/PS1 transgenic AD mice.
Publications

Scientific Journal reports

  1. Wu PF, Bhore N, Lee YL, Chou JY, Chen YW, Wu PY, Hsu WM, Lee H, Huang YS, Lu PJ, Liao YF. Phosphatidylinositol-4-phosphate 5-kinase type 1α attenuates Aβ production by promoting non-amyloidogenic processing of amyloid precursor protein [published online ahead of print, 2020 Jul 20]. FASEB J. 2020; 10.1096 /fj.202000113R. doi:10.1096/fj.202000113R (IF: 4.966, Biology 9/93)
  2. Wu PY, Yu IS, Lin YC, Chang YT, Chen CC, Lin KH, Tseng TH, Kargren M, Tai YL, Shen TL, Liu YL, Wang BJ, Chang CH, Chen WM, Juan HF, Huang SF, Chan YY, Liao YF, Hsu WM, and Lee H. Activation of aryl hydrocarbon receptor by kynurenine impairs the progression and metastasis of neuroblastoma. Cancer Res. 2019 Nov 1;79(21):5550-5562. doi: 10.1158/0008-5472. (IF: 8.378, Oncology 21/230)
  3. Wu PY, Chuang PY, Chan YY, Tsai TC, Wang BJ, Lin KH, Hsu WM, Liao YF, and Lee H. Novel Endogenous Ligands of Aryl Hydrocarbon Receptor Mediate Neural Development and Differentiation of Neuroblastoma. ACS Chem Neurosci. 2019 Sep 18;10(9):4031-4042. doi: 10.1021/acschemneuro.9b00273. (IF: 3.861, Chemistry, Medicinal 13/61)
  4. Wu PY*, Lin YC*, Huang YL, Chen WM, Chen CC, Lee H. Mechanisms of Lysophosphatidic Acid-Mediated Lymphangiogenesis in Prostate Cancer. Cancers (Basel). 2018 Oct 31;10(11). pii: E413. doi: 10.3390/cancers10110413. (IF: 6.162, Oncology 31/230) * Joint first author
  5. Wang BJ*, Wu PY*, Chen YW*, Chang YT, Bhore N, Wu PF, Liao YF. Quantitative measurement of γ-Secretase-mediated amyloid precursor protein and notch cleavage in cell-based luciferase reporter assay platforms. J Vis Exp. 2018 Jan 25;(131). doi: 10.3791/56795.(IF: 1.108, Multidisciplinary science 41/69)    * Joint first author
  6. Weng WC*, Lin KH*, Wu PY*, Ho YH, Liu YL, Wang BJ, Liao YF, Lee WT, Hsu WM, and Lee H. VEGF expression correlates with neuronal differentiation and predicts a favorable prognosis in patients with neuroblastoma. Sci Rep. 2017 Sep 11;7(1):11212. (IF: 4.011, Multidisciplinary science 15/69) * Joint first author
  7. Wang BJ, Her GM, Hu MK, Chen YW, Tung YT, Wu PY, Hsu WM, Lee H, Jin LW, Hwang SL, Chen RP, Huang CJ, Liao YF. ErbB2 regulates autophagic flux to modulate the proteostasis of APP-CTFs in Alzheimer's disease. Proc Natl Acad Sci U S A. 2017 Apr 11;114(15): E3129-E3138. (IF: 9.58, Multidisciplinary science 7/69)
  8. Hsu WM, Huang CC, Lee HY, Wu PY, Wu MT, Chuang HC, Lin LL, Chuang JH. MDA5 complements TLR3 in suppression of neuroblastoma. Oncotarget. 2015 Sep 22;6(28):24935-46.
  9. Weng WC, Lin KH, Wu PY, Lu YC, Weng YC, Wang BJ, Liao YF, Hsu WM, Lee WT, Lee H. Calreticulin Regulates VEGF-A in Neuroblastoma Cells. Mol Neurobiol. 2015 Aug;52(1):758-70. (IF: 4.586, Neuroscience 57/267)
  10. Kuo CT*, Chuang FT*, Wu PY*, Lin YC, Liu HK, Huang GS, Tsai TC, Chi CY, Wo AM, Lee H and Lee SC. Experimental demonstration of bindingless signal delivery in human cells via microfluidics. Journal of Applied Physics. 2014 Jul;116: 044702. (IF: 2.328, Physics, Applied 59/148) *Joint first author
  11. Lu YC, Chen CN, Chu CY, Lu J, Wang BJ, Chen CH, Huang MC, Lin TH, Pan CC, Chen SS, Hsu WM, Liao YF, Wu PY, Hsia HY, Chang CC, Lee H. Calreticulin activates β1 integrin via fucosylation by fucosyltransferase 1 in J82 human bladder cancer cells. Biochem J. 2014 May 15;460(1):69-78. (IF: 4.331, Biochemistry & Molecular Biology 73/299)
  12. Wu PY, Liao YF, Juan HF, Huang HC, Wang BJ, Liu YL, Yu IS, Shih YY, Jeng YM, Hsu WM, Lee H. Aryl hydrocarbon receptor downregulates MYCN expression and promotes cell differentiation of neuroblastoma. PLoS One. 2014 Feb 21;9(2): e88795. (IF: 2.776, Multidisciplinary science 24/69)
  13. Wu PY, Lin YC, Lan SY, Huang YL, Lee H. Aromatic hydrocarbon receptor inhibits lysophosphatidic acid-induced vascular endothelial growth factor-A expression in PC-3 prostate cancer cells. Biochem Biophys Res Commun. 2013 Aug 2;437(3):440-5. (IF: 2.705, Biochemistry & Molecular Biology 157/299)
  14. Hsu WM, Huang CC, Wu PY, Lee H, Huang MC, Tai MH, Chuang JH. Toll-like receptor 3 expression inhibits cell invasion and migration and predicts a favorable prognosis in neuroblastoma. Cancer Lett. 2013 Aug 19;336(2):338-46. (IF: 6.508, Oncology 29/230)
  15. Wang BJ, Wu PY, Lu YC, Chang CH, Lin YC, Tsai TC, Hsu MC, Lee H. Establishment of a cell-free bioassay for detecting dioxin-like compounds. Toxicol Mech Methods. 2013 Jul;23(6):464-70. (IF: 2.276, Toxicology 58/93)
  16. Lu MY, Liu YL, Chang HH, Jou ST, Yang YL, Lin KH, Lin DT, Lee YL, Lee H, Wu PY, Luo TY, Shen LH, Huang SF, Liao YF, Hsu WM>, Tzen KY; National Taiwan University Neuroblastoma Study Group. Characterization of neuroblastic tumors using 18F-FDOPA PET. J Nucl Med. 2013 Jan;54(1):42-9. (IF: 7.308, Radiology, Nuclear Medicine & Medical Imaging 5/129)
  17. Huang TC, Chang HY, Chen CY, Wu PY, Lee H, Liao YF, Hsu WM, Huang HC, Juan HF. Silencing of miR-124 induces neuroblastoma SK-N-SH cell differentiation, cell cycle arrest and apoptosis through promoting AHR and repressing MYCN. FEBS Lett. 2011 Nov 16;585(22):3582-6. (IF: 2.675, Biochemistry & Molecular Biology 157/299)

Abstracts:

  1. Wu PY, Chuang PY, Chan YY, Liao YF, Hsu WM and Lee H. The roles of endogenous ligand for aryl hydrocarbon receptor in neural development and tumorigenesis of neuroblastoma. ANR, 2018
  2. Lee H, Wu PY, Chan YY, Chuang PY, Liao YF, and Hsu WM. The roles of endogenous ligand for aryl hydrocarbon receptor in the neural development and the tumorigenesis of neuroblastoma. ASPN, 2017
  3. Wu PY, Lee CL, Shih YY, Hon JH, Hsu WM and Liao YF. Calreticulin regulates MYCN expression to control neuronal differentiation and stemness of neuroblastoma. ASPN, 2017
  4. Wu PY, Liao YF, Wen WC, Lin KH, Hsu WM, and Lee H Calreticulin-dependent VEGF expression promotes neuroblastoma differentiation. ANR, 2016
  5. Wu PY, Hsu WM, Lee H Aryl Hydrocarbon Receptor Suppresses Tumor Progression of Neuroblastoma. ANR, POB046, 2014
  6. Wu PY, Hsu WM, Lee H Aromatic Hydrocarbon Receptor Down-regulates MYCN Expression and Predicts Favorable Clinical Outcome of Neuroblastoma. ASCB, 63:713,2011
  7. Liu YL, Wu PY, Tzen KY, Jou ST, Lu MY, Yang YL, Chang HH, Lin DT, Lin KH, Hsu, WM, Lee, H Neuroblastic Tumors Demonstrating Stronger 18F-FDOPA Avidity on Positron Emission Tomography Had Higher Level of Aromatic Amino Acid Decarboxylase (DDC) Gene Expression. SIOP, 2011
  8. Hsu WM, Wu PY, Lee H Aromatic Hydrocarbon Receptor Down-regulates MYCN Expression and Promotes Neural Differentiation of Neuroblastoma. ANR, POC11, 2010
  9. Wu PY, Hsu WM, Lee H Aromatic Hydrocarbon Receptor Down-regulates MYCN Expression and Promotes Neural Differentiation of Neuroblastoma. FASEB, 23:740.15, 2009
  10. Wu PY, Hsu WM, Lee H Aromatic Hydrocarbon Receptor Down-regulates MYCN Expression and Promotes Neural Differentiation of Neuroblastoma. AEARU, P168, 2009
Matters
Honors
  1. 11/2016 Excellent Poster Award of Institute of Cellular and Organismic Biology, Academia Sinica.
  2. 11/2015 Best Poster Award of Asia-Pascific Symposium of Neuroblastoma
  3. 06/2013 Poster Award of Science Fair of College of Life science, NTU
  4. 06/2010 Poster Award of Translational Science- Animal Biotechnology Symposium
  5. 06/2010 NTU Teaching Assistant Award
  6. 02/2008 Taiwan Rotary Academic Scholarship
Laboratory
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